Author  
Place of duty  
Title   한국인의 비호지킨 림프종에서 Epstein-Barr Virus 검출의 의의 ( Significance of Epstein-Barr Virus Detection in non-Hodgkin's Lymphoma in Korea )
Publicationinfo   1997 Jan; 029(05): 851-867.
Key_word   Epstein-Barr virus, Non-Hodgkins lymphoma, Monoclonality, Lymphoma- genesis
Full-Text  
Abstract   Purpose: To investigate whether non-Hodgkins lymphoma of Korea is pathogenetically associated with Epstein-Barr virus(EBV). Materials and Methods: We analyzed fifty nine paraffin-embedded tissue and 22 fresh frozen tissue samples from non-Hodgkins lymphoma patients for the presence of EBV sequences by polymerase chain reactions(PCR), in situ hybridization(ISH) and assessed the clonality of EBV infected cells by Southern blot hybridization. Result: On ISH using oligonucleotide probes corresponding to EBV-encoded small RNAs (EBERs), 17(28.8%) of 59 paraffin-embedded tissue samples showed positive hybridization signals localized over the nuclei of the tumor cells, but PCR using primers from Internal Repeat I or EBV-determined nuclear antigen 1 gene showed positive results in only 6(10.2%) and 5(8.5%) samples, respectively. ISH and PCR did not detect EBV sequences in 15 paraffin-embedded tissue samples of tuberculous lymphadenitis patients. In 22 fresh frozen tissue samples, PCR detected EBV sequences in three samples from peripheral T cell lymphoma(PTCL). In two of those three samples, Southern blot analysis showed that these viral DNAs were monoclonal and of latent form. Conclusion: Approximately 28.8% of non-Hodgkins lymphoma were related to EBV in Korea. Monoclonality of those EBV DNAs implies that virus infection preceded malignant transformation, suggesting that EBV may play a role in lymphomagenesis.
저 자   서창인(Chang In Seo),김범준(Bum Joon Kim),박재원(Jae Won Park),황응수(Eung Soo Hwang),국윤호(Yoon Ho Kook),차창룡(Chang Ryong Cha)